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Pusa Su'esu'e ole Su'i Su'i ole Dengue Virus (PCR-fluorescence su'esu'ega)
Folasaga
O lenei pusa ua fa'amoemoe e su'esu'e lelei le ta'iina o le dengue virus nucleic acid i le toto o le tagata po'o le plasma fa'ata'ita'iga.O lenei pusa o loʻo faʻavae i luga o le vaega faʻapitoa i le genome atoa o le dengue virus type 1 ~ 4 e faʻataʻitaʻiina faʻamatalaga faʻapitoa ma TaqMan fluorescent probes mo ituaiga taʻitasi, ma iloa ai le vave iloa ma le taina o le dengue virus e ala i le PCR fluorescent taimi moni.
Parameter
* Ituaiga faʻataʻitaʻiga: Serum poʻo le Plasma * Mea faigaluega: ABI 7500 Real-Time PCR System;Bio-rad CFX96;Roche LightCycler480;SLAN PCR System. * Teuina -25 ℃ i le 8 ℃ e leʻi tatalaina ma puipuia mai le malamalama 18 masinaVaega 48T/Pusa Mea Autu DENV-Type tali paluga, lyophilized 2 paipa Primers, probes, PCR reaction buffer, dNTPs, Enzyme, ma isi. DENV lelei pulea, lyophilized 1 paipa Plasmids mo tandem dengue virus ituaiga 1-4 iloa vaega o lo'o taula'i Le pulea lelei (Suavai mama) 1.5ml Vai mama Tusi Taiala 1 iunite / Fa'atinoga
• Vave: Taimi pu'upu'u PCR fa'ateleina i oloa fa'apena.• Ma'ale'ale maualuga & fa'apitoa: Fa'alauiloa vave su'esu'ega mo togafitiga vave.• Malamalama lelei e tetee atu i le faalavelave.Laasaga o galuega
